Journal: Journal of visualized experiments : JoVE

Article Title: Reusable Single Cell for Iterative Epigenomic Analyses

doi: 10.3791/63456

Figure Lengend Snippet: Table 3

Article Snippet: NEST 2 mL 96-Well Deep Well Plate, V Bottom , Opentrons , N/A , 2 mL deep well 96-well plate.

Techniques: Staining, Fluorescence, Microscopy, Plasmid Preparation, Bioburden Testing, Clone Assay, Ligation, Cell Counting, Spectrophotometry, Purification, Electron Microscopy, Transferring, Gel Extraction, Picogreen Assay, Recombinant

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Duplication and transformation of the Schizosaccharomyces pombe collection of deletion strains

doi: 10.1007/978-1-4939-7546-4_18

Figure Lengend Snippet: Relative transformation efficiencies of pHL2882 in four deletion strains from the Bioneer Version 2.0 set using the 96-well format, lithium acetate transformation protocol. Four representative deletion strains (ΔRec25, Trm5Δ, ΔVp1302 and ΔMus7) are shown following transformation with a 5 day, 32°C grow-out on PMG-ura+leu+B1. Both the experimental plate (transformed with plasmid pHL2882) and the matched negative control (no DNA) are shown in parallel. Strains with relatively high (ΔRec25) and low (ΔMus7) transformation efficiency are shown to illustrate the range present in the deletion collection.

Article Snippet: Once revived on the single well rectangular plate, strains are then “re-pinned” from the non-selective revival plate, (again using the spring-loaded, long-pin “pinner” [VP 408FS2AS-1]) into sterile, “deep-well” 96-well plates [E&K Scientific, 2.2 mL, cat # EK-2440-S; See Note 14 ], in which each well contains 700 uL of YES G418 media. (See Note 15 ).

Techniques: Transformation Assay, Plasmid Preparation, Negative Control